Serum Annexin V and Anti-Annexin V levels and their relationship with metabolic parameters in patients with type 2 diabetes

SUMMARY BACKGROUND We investigated the serum annexin V and anti-annexin V levels and their relationship with metabolic parameters in patients recently diagnosed type 2 diabetic. METHODS A total of 143 patients recently diagnosed type 2 diabetes and 133 control subjects were included in the study. Body mass index (BMI), hs-CRP, HOMA-IR, carotid intima-media thickness, and serum levels of annexin V and anti-annexin V were investigated. RESULTS HOMA-IR, serum hs-CRP, and carotid intima-media thickness were found to be statistically significant. The Pearson correlation analysis revealed a statistically significant positive relationship between the carotid intima-media thickness and the annexin V level (r=0.29, p=0.006*). A statistically significant positive relationship was also detected between the Annexin V level and level of serum hs-CRP (r=0.29 p=0.006*). CONCLUSION A positive relationship was observed between the carotid intima-media thickness and annexin V at the end of our investigation. In this regard, we also believe that serum levels of annexin V may be increased for cardiovascular protection in the elevation of carotid intima-media thickness.

RESUMO OBJETIVO Investigar os níveis séricos de anexina V e antianexina V e sua relação com os parâmetros metabólicos em pacientes diabéticos tipo 2 recém-diagnosticados. MÉTODOS Foram incluídos no estudo 143 pacientes e 133 controles com diabetes tipo 2 recém-diagnosticado. O índice de massa corporal (IMC), PCR-as, Homa-IR, espessura íntima média carotídea e níveis séricos de anexina V e antianexina V foram investigados. RESULTADOS O Homa-IR, a PCR-s do soro e a espessura média da carótida foram estatisticamente significantes. A análise de correlação de Pearson revelou uma relação positiva estatisticamente significante entre a espessura média da carótida e anexina V (r=0,29; p=0,006 *). Foi também detectada uma relação positiva estatisticamente significativa entre o nível de anexina V e o nível sérico de PCR-as (r=0,29, p=0,006*). CONCLUSÃO Também foi observada uma relação positiva entre a espessura média da carótida e anexina V no final da nossa investigação. A esse respeito, também pensamos que os níveis séricos de anexina V podem ser aumentados para proteção cardiovascular na elevação da espessura média da carótida.

Niveles de anticuerpos anti-anexina A5 y manifestaciones de síndrome antifosfolípido primario obstétrico / Levels of anti-anexinal antibodies A5 and manifestations of obstetric primary antiphospholipid syndrome

El Síndrome Antifosfolípidos (SAF) describe un trastorno trombofílico autoinmune caracterizado por complicaciones obstétricas. La Anexina A5 (Anx A5) es una proteína que se estudia como un nuevo autoantígeno presente en el SAF, la presencia de autoanticuerpos frente a Anx A5 podría causar trombosis placentaria y pérdida del embarazo. El objetivo de este estudio fue analizar los niveles de IgG e IgM anti-Anx A5 en mujeres con SAF primario obstétrico y su asociación con diferentes complicaciones en una población de la ciudad de Córdoba. Se trabajó con muestras de pacientes puérperas que asistieron al Hospital Córdoba y al Hospital Materno Neonatal durante los años 2013-2017 con diagnóstico de SAF obstétrico y un grupo control formado por pacientes con embarazos normales. En la mayoría de las pacientes estudiadas, los niveles de IgG e IgM anti-Anx A5 se encontraron por debajo del rango de referencia, se mostró un aumento estadísticamente significativo de los niveles de IgG en pacientes con SAF respecto al grupo control. Pero no existieron asociaciones específicas entre los niveles de anticuerpo y los tres tipos de manifestaciones clínicas presentes en los criterios de clasificación. Estos hallazgos podrían sugerir una relación entre los anticuerpos anti-Anx A5 con el SAF obstétrico.

Antiphospholipid Syndrome (APS) describes an autoimmune thrombophilic disorder characterized by obstetric complications. Annexin A5 (Anx A5) is a protein that is studied as a new autoantigen present in APS, the presence of autoantibodies against Anx A5 could cause placental thrombosis and possibly pregnancy loss. The aim of this study was to analyze levels of IgG and IgM anti-Anx A5 in women with primary obstetric APS and its association with different complications in a population of the city of Córdoba. We worked with samples of puerperal patients who attended the Córdoba Hospital and the Maternal Neonatal Hospital during the years 2013-2017 with a diagnosis of obstetric APS and a control group formed by patients with normal pregnancies. In most of the patients studied, levels of IgG and IgM anti-Anx A5 were below the reference range, is demonstrate an increase statistically significant in the levels of the IgG in patients with APS compared with control group. But there were no specific associations between antibody levels and the three types of obstetric clinical manifestations present in the classification criteria. These findings could suggest a relationship between anti-Anx A5 antibodies and obstetric APS.

A Meta-Analysis of Circulating Microvesicles in Patients with Myocardial Infarction / Uma Meta-Análise de Microvesículas Circulantes em Pacientes com Infarto do Miocárdio

Abstract Background: Cell-derived microvesicles (MVs) are vesicles released from activated or apoptotic cells. However, the levels of MVs in myocardial infarction have been found inconsistent in researches. Objective: To assess the association between MVs and myocardial infarction by conducting a meta-analysis. Methods: A systematic literature search on PubMed, Embase, Cochran, Google Scholar electronic database was conducted. Comparison of the MVs levels between myocardial infarction patients and healthy persons were included in our study. Standard Mean Difference (SMD) and 95% confidence interval (CI) in groups were calculated and meta-analyzed. Results: 11 studies with a total of 436 participants were included. Compared with the health persons, AMVs [SMD = 3.65, 95% CI (1.03, 6.27)], PMVs [SMD = 2.88, 95% CI (1.82, 3.93),] and EMVs [SMD = 2.73, 95% CI (1.13, 4.34)], levels were higher in patients with myocardial infarction. However, LMVs levels [SMD = 0.73, 95% CI (-0.57, 2.03)] were not changed significantly in patients with myocardial infarction. Conclusions: AMVs, PMVs and EMVs might be potential biomarkers for myocardial infarction.

Resumo Fundamentos: As microvesículas derivadas de células (MVs) são vesículas liberadas de células ativadas ou apoptóticas. No entanto, os níveis de MVs no infarto do miocárdio foram encontrados inconsistentes nas pesquisas. Objetivo: Avaliar a associação entre MV e infarto do miocárdio por meio de uma meta-análise. Métodos: Foi realizada uma pesquisa sistemática na literatura em PubMed, Embase, Cochran e no banco de dados eletrônico do Google Scholar. Uma comparação dos níveis de MV entre pacientes com infarto do miocárdio e pessoas saudáveis foi incluída no nosso estudo. A Diferença Média Padrão (DMP) e o intervalo de confiança (IC) de 95% nos grupos foram calculadas e meta-analisadas. Resultados: Foram incluídos 11 estudos com um total de 436 participantes. Em comparação com as pessoas saudáveis, as MVA [DMP = 3,65, IC 95% (1,03, 6,27)], MVPs [DMP = 2,88, IC 95% (1,82, 3,93)] e MVEs [DMP = 2,73, IC 95% (1,13, 4.34)], foram maiores em pacientes com infarto do miocárdio. No entanto, os níveis de MVL [DMP = 0,73, IC 95% (-0,57, 2,03)] não foram alterados significativamente em pacientes com infarto do miocárdio. Conclusões: MVAs, MVPs e MVEs podem ser biomarcadores potenciais para o infarto do miocárdio.

Descripción de la presencia de anticuerpos antianexina V, cardiolipina y B2-glicoproteína I en mujeres con pérdidas gestacionales recurrentes en la ciudad de Medellín, Colombia / Describing the presence of anti-annexin V antibodies, cardiolipin and B2-glycoprotein 1 in females suffering recurrent pregnancy loss in Medellin, Colombia

Objetivo: describir la frecuencia de anticuerpos antianexina V en una serie de mujeres que presentaron aborto recurrente en la ciudad de Medellín. Materiales y métodos: se llevó a cabo una serie de casos constituida por mujeres sanas que acudían a consulta ginecológica por historia de pérdidas gestacionales recurrentes caracterizadas por dos o más abortos en el primer trimestre de gestación, en dos instituciones prestadoras de servicios de salud de segundo y tercer nivel de complejidad, y a la consulta privada de algunos ginecólogos de la ciudad de Medellín que aceptaron participar en el estudio. Se excluyeron las mujeres con diagnóstico de síndrome antifosfolípido secundario. Se evaluaron las características sociodemográficas, los antecedentes obstétricos y médicos con énfasis en hipertensión arterial y trombosis. También se evaluaron los anticuerpos anticardiolipina, anticoagulante lúpico, número de anticuerpos antiß2-GPI y número de anticuerpos antianexina V. La información se tabuló en una hoja de cálculo de Microsoft Excel y se procesó en el programa estadístico Stata 10.0, en el análisis estadístico se utilizó estadística descriptiva. Resultados: se encuestaron 65 mujeres con edades entre los 18 y 46 años, la mitad de las pacientes reportaron 2 abortos antes de la décima semana, y la otra mitad después de esta. En los resultados de los anticuerpos anticardiolipina se encontró que el 4,6% (3) de las pacientes presentaron anticuerpos positivos IgM y el 3,1% (2) tenían anticuerpos positivos IgG. El 3,1% (2) de las mujeres presentaron anticuerpos antiß2-GPI IgG e IgM. El 4,6% (3) de las pacientes presentaron anticuerpos positivos antianexina IgG y un 12,3% (8) de estas presentaron un resultado indeterminado.Conclusiones: se evidencia la presencia de anticuerpos antianexina en las pacientes estudiadas, aunque un subgrupo de mujeres presentó un resultado indeterminado; surge la hipótesis de que la anexina V podría ser un factor importante e independiente de los anticuerpos cardiolipina y β2-GPI en las pérdidas gestacionales recurrentes.

Objective: Describing anti-annexin V antibody frequency in a series of females suffering recurrent abortion in Medellin. Materials and methods: A series of cases was compiled, consisting of healthy females attending gynecological consultation who had a background of recurrent pregnancy loss (RPL), characterized by two or more abortions during the first three months of pregnancy. The females were attending a second- and third-level complexity healthcareproviding institution and/or private consultation with some gynecologists in Medellin who agreed to participate in the study. Females diagnosed with secondary antiphospholipid syndrome were excluded. Socio-demographic characteristics, obstetric and medical background emphasizing hypertension and thrombosis were evaluated, as were anti-cardiolipin antibodies, lupus anticoagulant, number of anti-â2-GPI antibodies and number of anti-annexin V antibodies. Such information was tabulated on a Microsoft Excel sheet and processed using Stata 10.0 statistical software; descriptive statistics were used for statistical analysis. Results: 65 females were surveyed, their ages ranging from 18 to 46 years. Half the patients reported 2 abortions before the tenth week and the other half following such time. Anti-cardiolipin antibody results revealed that 4.6% (3) of the patients proved positive for IgM antibodies and 3.1% (2) positive for IgG antibodies. 3.1% (2) of the females had IgG and IgM anti-â2-GPI antibodies. 4.6% (3) of the patients were positive for anti-annexin IgG antibodies and 12.3% (8) had an indeterminate result. Conclusions: Anti-annexin antibodies were detected in the patients surveyed in this study, although a subgroup of females had an indeterminate result. This led to advancing a hypothesis that annexin V could be an important and independent factor for cardiolipin and â2-GPI antibodies in recurrent pregnancy loss.Conclusions: Anti-annexin antibodies were detected in the patients surveyed in this study, although a subgroup of females had an indeterminate result. This led to advancing a hypothesis that annexin V could be an important and independent factor for cardiolipin and β2-GPI antibodies in recurrent pregnancy loss.